Validation of a Chemiluminescent Enzyme Immunometric Assay for Plasma Adrenocorticotropic Hormone in the Dog

Abstract

This chemiluminescent assay is a valid technique for measurement of ACTH in canine plasma. ACTH concentration measured by chemiluminescence is lower than that measured by immunoradiometry. Aprotinin decreases the measured concentration of ACTH, and this effect should be taken into account when interpreting results. Diluents supplied with the kit should not be used for dilution of canine samples.