Immunoassay of Staphylococcal Enterotoxin C1 by FTIR Spectroscopy and Electrochemical Gold Electrode

Abstract

A self-assembled gold surface acting as a substrate, a kind of antibody immunosensor is described. This sensor is composed of a three-electrode system and a layer of antibody of staphylococcal enterotoxin C1(SEC1). The antigen of SEC1 is detected by an electrochemical method at a constant antibody concentration. The redox probe is used to determine the extent of the biolayer insulation. AC impedance spectroscopy is fast and simple and it can be applied as an electrochemical sensing device for the antigen. Fourier transform IR spectroscopy is used to confirm the adsorption of the SEC1 antibody on the gold electrode and the association of SEC1 antigen and antibody.