Variants in clones of gene-machine-synthesized oligodeoxynucleotides

Abstract

We have found numerous sequence variants in clones of synthetic oligodeoxynucleotides containing tRNA genes. In four constructs, the frequency of variants was 8/20 or 40% per clone and 10/3152 or 0.3% per nucleotide (see table ). Other researchers have also observed variants in synthetic oligos; thus, care must be exercised with this technology.