Universal immuno-PCR for ultra-sensitive target proteindetection

Abstract

The exponential amplification power of polymerase chain reaction However, the limited availability of its key component, a streptavidin-protein A fusion protein, and the widely varied affinities of protein A with antibodies of various classes and subclasses from different species (3) limited its immediate applications. We have substituted the fusion protein with commercially available biotinylated secondary antibodies and free streptavidin, modified the sample dilution solution and reduced washing steps to a reasonable scale. The resulting procedure retains the sensitivity and specificity of the original Immuno-PCR, but can be universally applied without any Ig source or (sub)class restrictions.