Journal of the American Chemical Society · 2004 · 112 citations · 7 references
Life SciencesBiochemistryToxicologyBiochemical ToxicologyMechanisms Of ToxicityRedox BiochemistryAntioxidant DefenseMolecular Mechanisms Of ToxicityOxidative Damage To BiomoleculesOxidative StressOxidative Stress MechanismsReactive Oxygen SpeciesBufferingComputational Biology ModelsOxidative Defense SystemsOxidative Stress DefenseOxidative Stress In ToxicityOxidative Stress KineticsRedox BufferingRedox Buffering Capacity
The major antimicrobial products of neutrophilic myeloperoxidase (MPO) in physiologic fluids are hypochlorous acid (HOCl) and hypothiocyanite (OSCN-), and the former is generally believed to be the killing agent. However, we have determined that HOCl oxidizes SCN- in a facile nonenzymic reaction. The observed kinetics and computational models substantiate the hypothesis that SCN- serves to moderate the potential autotoxicity of HOCl by restricting its lifetime in physiologic fluids. Furthermore, the oxidizing equivalents of HOCl are preserved in OSCN-, a more discriminate biocide that is not lethal to mammalian cells.
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Normal Reference Laboratory Values
Alexander Kratz, Maryjane Ferraro, Patrick M. Sluss et al. · New England Journal of Medicine · 2004 · 593 citations
Dirk Roos, Christine C. Winterbourn · Science · 2002 · 171 citations