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Electrospray tandem mass spectrometry of poly(3-hydroxybutanoic acid) end groups analysis and fragmentation mechanism
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1998
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End Groups AnalysisEngineeringMacromolecular ChemistryBiological Mass SpectrometryRing-opening PolymerizationOrganic ChemistryAliphatic Polyester AnalysisChemistryPolymersAnalytical InstrumentationEnvironmental Analytical ChemistryBioanalysisAnalytical ChemistryPolymer ChemistryChromatographyNatural PhbFragmentation MechanismNatural PolymerBiochemistryBiopolymersBiomolecular EngineeringBiomanufacturing3-Hydroxybutanoic AcidMass SpectrometryProtein Mass SpectrometryPolymer CharacterizationMedicinePolymer SynthesisDrug Analysis
The electrospray ionization tandem mass spectrometry technique applied for the first time to aliphatic polyester analysis, allowed fast and reliable identification of the various macromolecules of poly(3-hydroxybutanoic acid) (PHB), synthesized via ring-opening polymerization of β-butyrolactone. It was demonstrated that the biomimetic polymers studied contain various end groups, depending on the anionic initiator employed, and show close similarity to the natural PHB produced by enzymes in living cells of prokaryotic and eukaryotic organisms. © 1998 John Wiley & Sons, Ltd.