Tissue Culture Studies in<i>Duboisia myoporoides</i>; 1. Plant Regeneration and Clonal Propagation by Stem Node Cultures

Abstract

High frequency plant regeneration is reported from nodal explant cultures of Duboisia myoporoides . For the optimal growth of the axillary bud and it subsequent multiplication, fortification of 1.0 mg.l -1 IAA along with 2.0 mg.l -1 Kn or BAP was found to be essential. No other auxin could replace IAA since they led to callus formation. Rooting could be induced by auxin alone (0.5 mg.l -1 NAA), as expected, but only after replacing semi-solid basal medium of Murashige and Skoog (MS) with static liquid MS. No synergism was noticed between cytokinins and adenine sulphate, while GA 3 proved significantly suppressive for the shoot growth. The plantlets thus obtained were successfully raised in large numbers to normal adults under field conditions