Reduction of variation in T‐cell subset enumeration among 55 laboratories using single‐platform, three or four‐color flow cytometry based on CD45 and SSC‐based gating of lymphocytes

Abstract

The state-of-the-art technology for T-cell subset enumeration was implemented successfully among 55 Belgian-Dutch laboratories and resulted in significant reductions of between-site variation of absolute CD3+, CD4+, and CD8+ T-cell counts.