Chitosan/heparin surface modified polyacrylic acid grafted polyurethane film by two step plasma treatment

Abstract

The aim of this study is to design an artificial skin dress. A multilayer skin dressing included synthesised castor oil based polyurethane (PU) as the outer layer and two biopolymeric layers of heparin and chitosan as the inner layers were prepared. The surface of PU film was activated using two steps oxygen radio frequency plasma treatment. The surface of the modified PU films characterised by attenuated total reflection Fourier transform infrared spectroscopy, scanning electron microscopy and water drop contact angle measurements. Scanning electron microscopy observations confirmed the presence of grafted poly acrylic acid on the surface of PU films. Also, heparin and chitosan were immobilised on PU films. In vitro cell culture showed that the samples have an excellent biocompatibility with L929 fibroblast cells. Cell adhesion and proliferation of cells on the chitosan/heparin immobilised surfaces showed better behaviours compared with poly (acrylic acid) grafted PU film.