Biologia Plantarum · 1988 · 12 citations · 5 references
Life SciencesPlant BiologyPlant SciencesEmbryogenesisEmbryologyPlant Developmental BiologyEmbryo DevelopmentFlower DevelopmentPollen BiologyPollen DevelopmentPollen ProductionSeed DevelopmentAnther CultureBud Differentiation ControlCulture Medium FormulationFloral Bud DifferentiationFlower Bud DifferentiationPollen CulturePollen Developmental PhysiologyPollen Embryology
Pollen embryogenesis occurred in anther cultures of two genotypes ofBrassica carinata A. Braun. Pretreatment of anthers at 35°C for 3 or 6 days was essential for the induction of androgenesis on growth regulator-free culture medium. A combination of sucrose and glucose was better than sucrose alone. None of the pollen embryos germinated normally. Full plants were raised through adventitious bud differentiation from their hypocotyl.
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