Determination of arsenic in biological samples by inductively coupled plasma mass spectrometry with selenium as an internal standard

Abstract

An internal standard method is described for the determination of arsenic in mussel and oyster samples by inductively coupled plasma mass spectrometry. 0.1 g of sample was digested in a microwave oven with addition of 1 mL of HNO3 and 1 mL of H2O2. 77Se was selected as an internal standard because 77Se was close to As in mass, the first ionization potential and ion-exchange behavior. In order to eliminate spectral interferences and matrix effects, the analyte was separated from matrix elements using AG2-X8 anion exchange resin. An internal standard method was used for quantification which adopted the isotope dilution procedure assuming 77Se as a pseudo-isotope of As. The proposed method was validated by analyzing mussel reference material (NIST SRM 2976). The determined As concentration of the reference material agreed well with the certified value within the uncertainty limit. The method was then applied to the analysis of an oyster reference material developed by Korea Research Institute of Standards and Science.