Monitoring Salivary Melatonin Concentrations in Children With Sleep Disorders Using Liquid Chromatography–Tandem Mass Spectrometry

Abstract

A validated liquid chromatography-tandem mass spectrometry method suitable for monitoring salivary melatonin in children with circadian rhythm sleep disorders is reported. The method also has potential application to pediatric population pharmacokinetic studies using sparse sampling of saliva as the biological sample matrix.