Bilirubin Oxidase and [Fe(CN)6]3−/4− Modified Electrode Allowing Diffusion-controlled Reduction of O2 to Water at pH 7.0

Abstract

Abstract An enzyme-modified electrode was prepared producing a diffusion-limited bioelectrocatalytic current for the reduction of O2 to water at neutral pH and at ambient temperature. The electrode used bilirubin oxidase as an enzyme and [Fe(CN)6]3−/4− as a mediator, both of which were immobilized on the surface of a glassy carbon electrode by electrostatic entrapment with poly-l-lysine.