Parasitology · 1993 · 22 citations · 19 references
AgricultureBiologyGeneticsParasitologyPlant GeneticsPlant NematologyCyst NematodesNematode BiologyNematode DetectionNematode DiagnosisNematode IdentificationPlant-parasitic NematodesAmplified Fragment Length Polymorphism MarkersDiagnostic Assay DevelopmentDiagnostic Assay ValidationDna Species IdentificationMolecular Diagnosis Of NematodesPolymerase Chain Reaction (Pcr)-based Nematode DetectionRandom Amplified Polymorphic Dna AnalysisRandom Amplified Polymorphic Dna Markers
Random amplified polymorphic DNA (RAPD) offers a potential basis for the development of a diagnostic assay to differentiate the potato cyst nematode species Globodera rostochiensis and G. pallida. Nine decamer primers have been tested for their ability to amplify species-specific DNA sequences. Primer OPG-05 produced 2 discrete DNA fragments, which were consistently present in 5 G. rostochiensis populations and absent in 5 G. pallida populations. These fragments were detectable in single females as well as in single 2nd-stage juveniles. Their amplification is extremely efficient, and reproducible over a wide range of template concentrations. One-fifth of a single juvenile is sufficient to generate reproducible RAPD markers. The amplification from single juveniles requires no DNA isolation. The use of a crude homogenate does not impair the polymerase chain reaction.
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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
John G. Williams, Anne R. Kubelik, Kenneth J. Livak et al. · Nucleic Acids Research · 1990 · 13.2K citations · Full text
Fingerprinting genomes using PCR with arbitrary primers
John Welsh, Michael McClelland · Nucleic Acids Research · 1990 · 5.2K citations · Full text
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li, Ulf Gyllensten, Xiangfeng Cui et al. · Nature · 1988 · 674 citations