PLANT PHYSIOLOGY · 1999 · 89 citations · 40 references
Life SciencesPlant BiologyPlant SciencesGene ExpressionMetabolismPlant BiochemistryCytochrome P450 EnzymesPhenylpropanoid BiosynthesisPhenylpropanoid MetabolismPlant EnzymesPlant Gene ExpressionPlant MetabolismC4 Pathway EnzymesCinnamate 4-HydroxylaseCinnamic Acid MetabolismCytochrome P450 Enzymes In Phenylpropanoid MetabolismPhenylalanine Ammonia-lyase PathwayPhenylalanine MetabolismPhenylpropanoid Pathway RegulationSequence Similarity
A previously isolated parsley (Petroselinum crispum) cDNA with high sequence similarity to cinnamate 4-hydroxylase (C4H) cDNAs from several plant sources was expressed in yeast (Saccharomyces cerevisiae) containing a plant NADPH:cytochrome P450 oxidoreductase and verified as encoding a functional C4H (CYP73A10). Low genomic complexity and the occurrence of a single type of cDNA suggest the existence of only one C4H gene in parsley. The encoded mRNA and protein, in contrast to those of a functionally related NADPH:cytochrome P450 oxidoreductase, were strictly coregulated with phenylalanine ammonia-lyase mRNA and protein, respectively, as demonstrated by coinduction under various conditions and colocalization in situ in cross-sections from several different parsley tissues. These results support the hypothesis that the genes encoding the core reactions of phenylpropanoid metabolism form a tight regulatory unit.
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