Infrared Photoactivation Reduces Peptide Folding and Hydrogen‐Atom Migration following ETD Tandem Mass Spectrometry

Abstract

Peptides see the light: The photoactivation of peptide precursor cations during electron transfer dissociation (ETD) led to the generation of isotopic cluster peaks that more closely resembled theoretically predicted product-ion distributions. This method should enable the application of ETD to low-charge-density peptide precursors, the gas-phase secondary structure of which prevents the direct formation of c- and z.-type fragment ions. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.