Microscopy Research and Technique · 2007 · 49 citations · 9 references
Natural SciencesBiomedical EngineeringBiophysicsBiomedical ImagingBiomedical OpticsDiagnostic ImagingFluorescence ImagingFluorescence MicroscopyFluorescence SpectroscopyHyperspectral ImagingOptical ImagingOptical SpectroscopyFluorescence LifetimeFluorescence Lifetime ImagingFluorescence Lifetime Imaging MicroscopyFluorescence Lifetime Imaging Of TissueFluorescence Lifetime MeasurementsFluorescence Microscopy AcquisitionMicroscope ImagingTime-resolved Fluorescence Imaging
We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high-speed FLIM technology in a line-scanning microscope. We demonstrate the acquisition of whole-field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue.
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Hyperspectral imaging: A novel approach for microscopic analysis
Roger A. Schultz, Thomas Nielsen, Jeff R. Zavaleta et al. · Cytometry · 2001 · 204 citations · Full text
Natural Sciences, Biomedical Engineering, Biomedical Imaging +16
Temporally and Spectrally Resolved Imaging Microscopy of Lanthanide Chelates
György Vereb, Elizabeth A. Jares‐Erijman, Paul R. Selvin et al. · Biophysical Journal · 1998 · 167 citations · Full text
Fluorescence lifetime imaging with picosecond resolution for biomedical applications
K. Dowling, Mark J. Dayel, M. J. Lever et al. · Optics Letters · 1998 · 145 citations