<i>Arabidopsis</i> gp91 ^phox homologues <i>AtrbohD</i> and <i>AtrbohF</i> are required for accumulation of reactive oxygen intermediates in the plant defense response

TLDR

Reactive oxygen intermediates are linked to plant defense, but their origin is debated, with Arabidopsis rboh genes suggested as key producers. The study used dSpm insertion mutants of AtrbohD and AtrbohF and challenged them with Pseudomonas syringae pv. tomato DC3000( avrRpm1 ) and the oomycete Peronospora parasitica to assess ROI production.

Abstract

Reactive oxygen intermediates (ROI) are strongly associated with plant defense responses. The origin of these ROI has been controversial. Arabidopsis respiratory burst oxidase homologues ( rboh genes) have been proposed to play a role in ROI generation. We analyzed lines carrying dSpm insertions in the highly expressed AtrbohD and AtrbohF genes. Both are required for full ROI production observed during incompatible interactions with the bacterial pathogen Pseudomonas syringae pv. tomato DC3000( avrRpm1 ) and the oomycete parasite Peronospora parasitica . We also observed reduced cell death, visualized by trypan blue stain and reduced electrolyte leakage, in the Atrboh mutants after DC3000( avrRpm1 ) inoculation. However, enhanced cell death is observed after infection of mutant lines with P. parasitica . Paradoxically, although atrbohD mutation eliminated the majority of total ROI production, atrbohF mutation exhibited the strongest effect on cell death.