Growth and fruitbody formation of Ganoderma lucidum on media supplemented with vanadium, selenium and germanium

Abstract

Responses of mycelia of Ganoderma lucidum to vanadium, selenium and germanium were examined over a wide range of concentrations (10-1,120 μg/ml) in pure culture. Se and V were found to be highly toxic, but Ge was not toxic at the levels tested. Ganododerma lucidum cultivated on substrates of sawdust with V (30-80/μg/g) developed mature fruitbodies, but the bioaccumulation of V was quite low (2.5-7 μg/g in pileus, 12.5-21.5 Fg/g in stipe and < 1 μg/g in basidiospores). Se as Na2Se04 labeled with 75Se was effectively taken up from substrates and accumulated in fruitbodies (mainly in pileus), then depleted by discharge of basidiospores. Ge as GeCI4 labeled with 77Ge was easily uptaken and translocated into fruitbodies.