Studies on the DNA Fragments of Mammals and Drosophila Containing Structural Genes and Adjacent Sequences

Abstract

Several hypothetical models have been proposed to explain the structure and function of the genome in eukaryotic cells (Scherrer and Marcaud 1968; Georgiev 1969; Britten and Davidson 1969; Crick 1971; Georgiev et al. 1972, 1974). However, there has been no experimental approach for the direct verification of these models. Now it becomes possible due to the techniques of cloning and amplification of fragments of eukaryotic DNA in bacteria (Morrow et al. 1974; Thomas et al. 1974; Wensink et al. 1974). Work in this direction has been started in our laboratory along two lines. First, among λgt-Dm clones containing random fragments of Drosophila melanogaster BNA, those containing structural genes were selected and the properties of these inserted regions were studied (Ilyin et al. 1976; Georgiev et al. 1977).