Neural tube defects induced by folate deficiency in mutant <i>curly tail</i> (<i>Grhl3</i>) embryos are associated with alteration in folate one‐carbon metabolism but are unlikely to result from diminished methylation

Abstract

Curly tail fibroblasts and embryos, in which Grhl3 expression is reduced, display alterations in one-carbon metabolism, particularly in the response to folate deficiency, compared to genetically matched congenic controls in which Grhl3 is unaffected. However, unlike folate deficiency, diminished methylation potential appears to be insufficient to cause cranial NTDs in the curly tail strain, nor does it increase the frequency of caudal NTDs.