Cloning of the β‐phycocyanin gene from <i>Anacystis nidulans</i>

Abstract

The β‐phycocyanin gene, pcyB , of Anacystis nidulans was isolated from an Escherichia coli λ‐phage bank by the use of synthetic oligonucleotides derived from the 170 amino acid sequence of the β‐phycocyanin protein. Two positive, overlapping λ‐clones were found. Partial DNA sequencing of one of the clones gave a deduced amino acid sequence which was in full agreement with a portion of the published sequence of A. nidulans β‐phycocyanin. A comparison with the published DNA sequence for β‐phycocyanin from Agmenellum quadruplicatum shows a DNA sequence homology of 70.4% over the sequenced region.