Plasma Inhibin Levels in Relation to Steroids and Gonadotrophins during Oestrous Cycle in Buffalo

Abstract

The study was conducted on six Murrah buffalo synchronized and induced to oestrus. An indwelling catheter was placed in the jugular vein of each buffalo 4 days before the expected onset of the oestrus following the induced oestrus and blood samples were collected at 8 h intervals from each animal throughout the oestrous cycle. Plasma immunoreactive inhibin, follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol-17 beta and progesterone were estimated by radioimmunoassay to study the variations in the peripheral levels of these hormones and their inter-relationships in order to elucidate the feedback systems controlling them during the oestrous cycle of buffalo. Plasma inhibin levels ranged between 391.25 and 631.97 pg/ml during various phases of the oestrous cycle and were found to be higher than reported in cows. Peak LH and FSH levels during oestrus were 38.40 +/- 9.21 and 24.04 +/- 4.75 ng/ml, respectively and estradiol-17 beta and progesterone were 19.50 +/- 5.51 pg/ml and 0.61 +/- 0.25 ng/ml, respectively. The mean plasma inhibin concentration on the day of oestrus was 562.5 +/- 18.9 pg/ml. Levels of FSH in the plasma showed three mid-cycle elevations which corresponded to comparatively lower inhibin and elevated estradiol-17 beta levels during the same period. From this observation it was deduced that both inhibin and estradiol-17 beta have a feed-back regulatory effect on FSH secretion in buffalo.