Immunological Detection and Isolation of a New Taxoid from the Stem Bark of <i>Taxus baccata</i>

Abstract

The separation of a stem-bark extract of Taxus baccata L. cv. stricta by HSCCC resulted in several chromatographic fractions that were analyzed by ELISA using specific anti-10-deacetylbaccatin III antibodies. In addition to 10-deacetylbaccatin III and to four known xylosyl taxoids, a new taxoid, 9-deacetyl-9-benzoyl-10-debenzoyltaxchinin A (1), was isolated, and its structure was elucidated using spectroscopic methods. The cross-reactivity of 1 with anti-10-deacetylbaccatin III antibodies was examined.