Journal of Virology · 2013 · 41 citations · 49 references
Life SciencesInfectious DiseasesMolecular BiologyHost-pathogen InteractionsMolecular VirologyVirologyAnimal VirusesInterferon ResponsesPathogenesis Of Infectious DiseaseViral Hemorrhagic FeversViral InfectionsViral PathogenesisAnimal Viral PathogenesisExperimental Animal Models Of Viral InfectionHemorrhagic Viral DiseaseHemorrhagic Viral FeversInterferon Response To Viral InfectionMurine ModelsNeuroviral PathogenesisType I Interferon Responses
Machupo virus (MACV) is the etiological agent of Bolivian hemorrhagic fever (BHF), a reemerging and neglected tropical disease associated with high mortality. The prototypical strain of MACV, Carvallo, was isolated from a human patient in 1963, but minimal in vitro and in vivo characterization has been reported. To this end, we utilized reverse genetics to rescue a pathogenic MACV from cloned cDNAs. The recombinant MACV (rMACV) had in vitro growth properties similar to those of the parental MACV. Both viruses caused similar disease development in alpha/beta and gamma interferon receptor knockout mice, including neurological disease development and high mortality. In addition, we have identified a novel murine model with mortality and neurological disease similar to BHF disease reported in humans and nonhuman primates.
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Clinical Virology of Lassa Fever in Hospitalized Patients
Karl M. Johnson, Joseph B. McCormick, P. A. Webb et al. · The Journal of Infectious Diseases · 1987 · 260 citations