Production and characterization of a monoclonal antitubulin antibody

Abstract

Abstract A permanent hybridoma that secretes monoclonal antibody to tubulin has been developed. Monoclonal antibody belongs to the IgG1 subclass and its specificity was determined by enzymoimmunoassay and by indirect immunofluorescence. A cytoplasmic microtubule complex was detected by this monoclonal antibody as demonstrated in three different cell types.