Evidence that the <i>trans</i>‐10,<i>cis</i>‐12 isomer of conjugated linoleic acid induces body composition changes in mice

TLDR

The study examined how enriched preparations of cis‑9, trans‑11 and trans‑10, cis‑12 conjugated linoleic acid (CLA) affect body composition in mice. The authors used these enriched CLA preparations to assess changes in body composition in a mouse model. Feeding mice the trans‑10, cis‑12 CLA isomer lowered body fat while increasing body water, protein, and ash, and in 3T3‑L1 adipocytes it reduced lipoprotein lipase activity and intracellular triacylglycerol and glycerol while boosting glycerol release, whereas the cis‑9, trans‑11 and trans‑9, trans‑11 isomers had no such effects, indicating that the observed body composition changes are attributable to the trans‑10, cis‑12 isomer.

Abstract

Abstract We investigated the effects of conjugated linoleic acid (CLA) preparations, which were enriched for the cis ‐9, trans ‐11 CLA isomer or the trans ‐10, cis ‐12 CLA isomer, on body composition in mice. Body composition changes (reduced body fat, enhanced body water, enhanced body protein, and enhanced body ash) were associated with feeding the trans ‐10, cis ‐12 CLA isomer. In cultured 3T3‐L1 adipocytes, the trans ‐10, cis ‐12 isomer reduced lipoprotein lipase activity, intracellular triacylglycerol and glycerol, and enhanced glycerol release into the medium. By contrast, the cis ‐9, trans ‐11 and trans ‐9, trans ‐11 CLA isomers did not affect these biochemical activities. We conclude that CLA‐associated body composition change results from feeding the trans ‐10, cis ‐12 isomer.