Utilization of bound lactase in clinical chemistry

Abstract

Abstract A “stick test” for the detection of lactose has been developed based upon the enzyme lactase chemically bound via Procion brilliant orange to DEAE‐cellulose. Utilization of the bound rather than the free enzyme allows an ease in its addition to and removal from the reaction medium as well as a facile control of reagent quality and quantity. The bound enzyme “stick” may be reused for several determinations.