Separation and Identification of Some Cephalosporins on Impregnated TLC Plates

Abstract

Ethylenediamine tetra-acetic acid disodium salt dihydrate was used as an impregnating reagent to resolve some cephalosporins. Both changes in the concentration of the impregnating reagent and in the composition of the mobile phase influenced the resolution. Three new solvent systems (I) propionic acid:2-propanol:water (6:3:3, v/v/v), (II) 2-prapanol:water:ethyl acetate (5:3:3, v/v/v) and (III) n-butanol:water:acetic acid (5:4:2, v/v/v) were found to be successful.