Evidence for an evolutionary relationship between the large adaptor nucleoporin Nup192 and karyopherins

Abstract

Significance Nuclear pore complexes (NPCs) are proteinaceous transport channels gating transport of macromolecules across the nuclear envelope. Nup192 is one of five members of an adaptor nucleoporin complex (ANC) that is believed to be integral for linking the NPC subcomplexes and accommodating the dilations of the central transport channel. We present the structure of the N-terminal half of Nup192 that uncovers similarities with karyopherins, suggesting an evolutionary relationship. Using biochemical and in vivo data, we generated an interaction map of the ANC, providing a starting point for its mechanistic dissection.