Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. 1985.

TLDR

Recombinant DNA advances enable molecular analysis and prenatal diagnosis of genetic diseases, including sickle cell anemia caused by a single base substitution in the beta‑globin gene. The study aims to develop a prenatal diagnostic assay for sickle cell anemia using enzymatic amplification of beta‑globin sequences and restriction site analysis. Fetal DNA from amniocentesis or chorionic villus sampling is digested with restriction enzymes, separated by electrophoresis, transferred to a membrane, and hybridized with cloned gene or oligonucleotide probes.

Abstract

Recent advances in recombinant DNA technology have made possible the molecular analysis and prenatal diagnosis of several human genetic diseases. Fetal DNA obtained by aminocentesis or chorionic villus sampling can be analyzed by restriction enzyme digestion, with subsequent electrophoresis, Southern transfer, and specific hybridization to cloned gene or oligonucleotide probes. With This disease results from homozygosity of the sickle-cell allele (rS) at the 3globin gene locus. The S allele differs from the wild-type allele (3A) by substitution of an A in the wild-type to a T at the second position of the sixth codon of the p chain gene, resulting in the replacement of a glutamic acid by a valine in the expressed protein. For the prenatal diagnosis of sickle cell anemia, DNA ob-