Memórias do Instituto Oswaldo Cruz · 2003 · 72 citations · 28 references
Veterinary MedicineMolecular BiologyMolecular GeneticsDiagnostic ParasitologyParasite TaxonomyParasitologyHelminth DiagnosticsLarval Stage IdentificationMolecular Diagnosis Of ParasitesParasitic Disease DiagnosisParasitic Disease PathogenesisParasitic PathologyDifferentiationEnzymesLarval Species IdentificationMolecular Detection Of ParasitesMolecular Diagnosis Of Parasitic InfectionsMolecular Species IdentificationParasitic Worm IdentificationParasitic Zoonotic Diseases
Angiostrongylus cantonensis, A. costaricensis, and A. vasorum are etiologic agents of human parasitic diseases. Their identification, at present, is only possible by examining the adult worm after a 40-day period following infection of vertebrate hosts with the third-stage larvae. In order to obtain a diagnostic tool to differentiate larvae and adult worm from the three referred species, polymerase chain reaction-restriction fragment length polymorphism was carried out. The rDNA second internal transcribed spacer (ITS2) and mtDNA cytochrome oxidase I regions were amplified, followed by digestion of fragments with the restriction enzymes RsaI, HapII, AluI, HaeIII, DdeI and ClaI. The enzymes RsaI and ClaI exhibited the most discriminating profiles for the differentiation of the regions COI of mtDNA and ITS2 of rDNA respectively. The methodology using such regions proved to be efficient for the specific differentiation of the three species of Angiostrongylus under study.
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Rapid evolution of animal mitochondrial DNA.
Wesley M. Brown, M George, Alex C. C. Wilson · Proceedings of the National Academy of Sciences · 1979 · 3.5K citations · Full text
Life Sciences, Biology, Genetics +13
Rapid sequencing of rDNA from single worms and eggs of parasitic helminths
Robin B. Gasser, Neil B. Chilton, Hervé Hoste et al. · Nucleic Acids Research · 1993 · 551 citations