Cancer · 1988 · 57 citations · 32 references
MedicineOncologySurgeryNeuro-oncologyPediatric OncologySurgical OncologyAneuploidyBrain TumorsCraniospinal IrradiationPediatric Brain TumorsPediatric Neuro-oncologyPediatric TumorsAneuploidy In Pediatric TumorsCancer AneuploidyCytological Ploidy AssessmentFlow Cytometric AnalysisFlow Cytometry Ploidy TestingMedulloblastoma IrradiationPediatric Central Nervous System TumorsPediatric Craniospinal Irradiation
Paraffin-embedded surgical specimens from 26 infants and children with medulloblastomas treated between 1972 and 1981 were examined for DNA ploidy by flow cytometry (FCM). All patients received a standard treatment (a combination of maximum debulking of medulloblastoma and postoperative craniospinal irradiation with a posterior fossa boost of 5000 rad or more). They were studied to correlate the results of the findings of FCM DNA analysis with their final outcome, DNA ploidy, and extent of tumor resection. All seven patients with totally resected aneuploid medulloblastoma are alive, whereas only one of six patients with subtotally resected diploid medulloblastoma is alive (P = 0.0047). The current study suggests both DNA ploidy and extent of surgical resection are the most important determinant of patients' prognosis. Patients in selected group, particularly those with subtotally resected diploid tumor, are advised to undergo aggressive adjuvant chemotherapy.
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Flow cytometry in clinical cancer research.
Barthel Barlogie, Martin N. Raber, Johannes Schumann et al. · PubMed · 1983 · 766 citations