Effects of Acute Restraint Stress on Sperm Motility and Secretion of Pituitary, Adrenocortical and Gonadal Hormones in Adult Male Rats

Abstract

The effects of acute restraint stress on sperm motility and reproductive endocrinology were investigated in adult rats. Sperm motility was determined by computer-assisted sperm analysis. Acute restraint stress reduced sperm motility starting after 30 min, and the sperm motility parameters, percentage of motile spermatozoa (%), straight-line velocity, curvilinear velocity, deviation of the sperm head from the mean trajectory and the maximum amplitude of lateral head displacement decreased. It also induced a significant elevation in plasma adrenocorticotropic hormone, prolactin, corticosterone and progesterone and decreased follicle-stimulating hormone, luteinizing hormone, testosterone and immunoreactive (ir-) inhibin. These results clearly demonstrated that the acute restraint stress rapidly suppressed sperm motility and increased the activity of the hypothalamus-pituitary-adrenal axis, whereas it disturbed hypothalamus- pituitary-gonadal axis activity.