Journal of Applied Bacteriology · 1986 · 25 citations · 10 references
Life SciencesBiochemistryMicrobiologyEnzymologyFood MicrobiologyMicrobial MetabolismEnzyme CharacterizationEnzyme IsolationEnzyme ProductionLactic Acid BacteriaMicrobial Enzyme ProductionMicrobial GrowthAmylase ProductionColumn ChromatographyCrystalline StructureEnzyme Production From Vegetable WastesFermentation Of Vegetable WastesGel ElectrophoresisMicrobial Polysaccharide ProductionMolecular Weight Determination
An organism producing α‐amylase and identified as Lactobacillus cellobiosus D‐39 was recently isolated from vegetable wastes. Its amylase was purified by (NH 4 ) 2 SO 4 precipitation and DEAE cellulose column chromatography and was obtained in crystalline form. It was fairly stable at a broadly neutral pH range and maximally active at 50°C. The molecular weight was 22 500 daltons as determined by SDS gel electrophoresis.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Ulrich K. Laemmli · Nature · 1970 · 251.6K citations