High performance liquid affinity chromatography (HPLAC) and its application to the separation of enzymes and antigens

Abstract

High performance liquid chromatography (HPLC) has seen a spectacular development during the last few years due to its rapidity and its high resolving power [l-7] . Recently, HPLC has also been applied to include biological macromolecules such as polynucleotides, polysaccharides and proteins [3-71. This paper describes the first use of bioaffinity supports (in contrast to normally used 'non-biological' stationary phases) to the resolution of biological macromolecules in combination with the technique of HPLC, and for this novel technique we suggest the term: high performance liquid affinity chromatography, HPLAC.