Angewandte Chemie International Edition · 2011 · 121 citations · 31 references
Life SciencesBiologyMolecular BiologyMolecular ImagingMolecular VirologyVirologyFluorescence ImagingFluorescence MicroscopyFluorescence SpectroscopyMolecular Probe DevelopmentSingle-molecule ImagingViral Rna BiologyCellular Fluorescence ImagingFluorescence Imaging ProbesFluorescent Nucleic Acid ProbesFluorescent ProbesLive-cell Imaging ProbesSignal-to-background EnhancementSignal-to-background RatioSignal-to-noise Ratio
A message from the virus was detected by a peptide nucleic acid probe that contains thiazole orange as a fluorescent base surrogate (see picture). The high specificity and biostability of the probes lead to significant improvements to the signal-to-background ratio in imaging the mRNA from the influenza H1N1 virus in living infected cells. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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