Detection of Kinase Activity Using Versatile Fluorescence Quencher Probes

Abstract

Turn off the light! A kinase assay system employs fluorescent peptides and a phosphate-selective fluorescence quencher probe, and can be placed on a microfluidic chip (see picture). The system can be used for real-time kinase monitoring, kinase inhibitor screening, and cancer diagnosis based on abnormal kinase activity observed in patients' samples. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.