Preparation of Bovine Immunoglobulins and Free Secretory Component and Their Specific Antisera

Abstract

The preparation of bovine IgG1, IgG2, IgM, secretory IgA and free secretolT component is described. The methods require a minimum of equipment and utilize four basic procedures: precipitation, electrophoresis, gel filtration, and DEAE chromatography. The electrophoretie and immunochemical techniques described for assaying the purity of the various preparations are sensitive and those utilizing radial or immunodiffusion are inexpensive. The immunoelectrophoretic and disc electrophoretic patterns for each preparation are diagnostic and highly reproducible. Methods: are described for the preparation of monospecifie antisera against each preparation and a polyvalent antiserum against bovine immunoglobulins.