Transmission of Plasmodium knowlesi by Anopheles stephensi

Abstract

(1) A virulent strain of Plasmodium knowlesi has been transmitted through Anopheles stephensi (four times) and A. maculipennis atroparvus mosquitoes (once). A second strain has been transmitted through A. stephensi once. (2) Oocysts developed on the midgut of the mosquitoes, but the sporozoites in the salivary glands were always scanty. (3) The shortest pre-patent period observed was 6.8 days. (4) There was no obvious change of virulence as a result of mosquito transmission.