Monolayer Cultures of Trypsinized Monkey Kidney Cells in Synthetic Medium. Application to Poliovirus Synthesis.

Abstract

SummaryA synthetic medium containing only cysteine, iso-leucine, d-ribose in a salt-glucose medium, buffered with Tris (hydroxy-methyl aminome thane) supports the outgrowth of monkey kidney cells. The outgrowth is comparable in both rate and amount with the lactalbumin hydrolysate-calf serum (M) medium and supports the synthesis of poliovirus. Propagation of poliovirus throughout a culture in either liquid or agar medium occurs more rapidly in the presence of a simple amino acid supplement than in the serum-containing medium. Cysteine can completely replace the amino acid supplement for propagation of Type 1 in liquid cultures.