Biosynthesized gold nanoparticle developed as a tool for detection of HCG hormone in pregnant women urine sample

Abstract

Biosynthesized gold nanoparticles is one of the novel benchmark for using medical commercial industry. Recently gold nanoparticles were used for the detection of tumor, hormones, glucose and other biomolecules in health care sectors. Due to the different functional properties AuNPs was used for hormone detection was carried out in this study. Biosynthesized gold nanoparticles were used to qualitatively determine the hCG hormone in pregnancy women urine sample. The biosynthesis of gold nanoparticles using C.nudiflora plant extract. Synthesized gold nanoparticles were characterized by different analytical techniques such as FESEM, XRD and FTIR. The synthesized gold nanoparticles was used to perform hCG hormone detection on both pregnancy positive and negative urine sample.500 μL of the prepared gold solution and an equal volume of the test sample were mixed and used for assays. After that, the solution was tested using a pregnancy test strip. The mixture of gold nanoparticles in the urine sample changed color into pink that confirmed pregnancy. Otherwise it turned into gray color that showed the absence of pregnancy. The method was supported 100% accurate in diagnosis of pregnancy and it may be a good alternative method for urine pregnancy test.