Biosynthesis of the Brain‐specific 14‐3‐2 Protein in a Cell‐free System from Wheat Germ Extract Directed with Poly(A)‐containing RNA from Rat Brain

Abstract

Abstract: 14‐3‐2 Protein is a neuron‐specific protein with a molecular weight of 46,000. Poly(A)‐containing RNA was prepared from free polysomes of rat whole brains by means of phenol‐chloroform extraction and oligo (dT)‐cellulose chromatography. This RNA directed the synthesis of 14‐3‐2 protein in a cell‐free, protein‐synthesizing system derived from wheat germ. 14‐3‐2 Protein was not detected in the products of endogenous incorporation and the products directed with liver poly(A)‐containing RNA. These results indicate that mRNA for 14‐3‐2 protein contains the poly(A) sequence and resides only in the brain.