High performance displacement chromatography: Calculation and experimental verification of zone development

Abstract

Abstract Effluent concentration profiles from high performance displacement chromatography were calculated by using the theory of equilibrium chromatography of Helfferich and Klein (1970). The close agreement between predictions of the theory neglecting mass transfer resistances and experimental results demonstrates that conditions close to ideal chromatography are attainable in HPLC. Individual adsorption isotherms, as measured by frontal development, closely followed Langmuirian behavior.