Development of a Monoclonal Antibody for Detection of<i>Leptosphaeria korrae,</i>the Causal Agent of Necrotic Ringspot Disease of Turfgrass

Abstract

Monoclonal antibody (MAb) LKc50 was developed against Leptosphaeria korrae strain ATCC 56289. The antibody was capable of detecting L. korrae from cultures and in naturally infected Kentucky bluegrass samples from three states. In cross-reactivity tests using indirect ELISA, MAb LKc50 reacted positively to all 24 isolates of L. korrae screened, including strains from six states, from both Kentucky bluegrass and bermudagrass. MAb LKc50 reacted negatively to 38 of 42 isolates of related and nonrelated fungi and negatively to apparently healthy grass (...)