Organic Letters · 2006 · 26 citations · 22 references
MedicineMedicinal ChemistryPharmacologyCancer PharmacologyChemotherapyNatural Product BiosynthesisAntitumor AntibioticsCell-free Synthetic BiologyCofactor BiosynthesisCofactor EngineeringCofactor-dependent BiosynthesisAntitumor Antibiotic BiosynthesisCell-free Biosynthetic Pathway DesignCell-free Transcription-translation SystemsCofactor DependenceReconstituted Cell-free SystemsSubstrate Limitation
[reaction: see text] Azinomycins have potential therapeutic value as antitumor agents; however, their biosynthesis is poorly understood. Here, we provide the first demonstration of a protein cell-free system capable of supporting complete in vitro biosynthesis of the antitumor agent azinomycin B. The cell-free system is utilized to probe the cofactor dependence and substrate requirements of the pathway en route to azinomycin.
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