Bioscience Biotechnology and Biochemistry · 1997 · 23 citations · 0 references
Life SciencesBiochemistryPharmacologyCellular MetabolismMetabolismRenal PathophysiologyAntioxidant DefenseMechanisms Of Toxic InjuryOxidative Damage To BiomoleculesOxidative StressOxidative Stress MechanismsReactive Oxygen SpeciesCellular Response To Oxidative StressHypoxic MicroenvironmentOxidative Stress In EpitheliumOxidative Stress In ToxicityOxidative Stress InjuryRenal Antioxidant DefenseRenal Oxidative StressTannin Composition
When cells from a cultured renal epithelial cell line, LLC-PK1, were cultured under hypoxic conditions (oxygen concentration of 2% or less) before reoxygenation was applied (95% air, 5% CO2), the leakage of lactate dehydrogenase (LDH) into the medium increased. This phenomenon was inhibited in the presence of dimethyl sulfoxide, a hydroxyl radical scavenger, suggesting the involvement of free radicals. Such oxidative stress was significantly inhibited by a green tea extract, and more potently by a tannin mixture. On the other hand, under ordinary culture conditions (95%, air, 5% CO2), there was cell injury, although the LDH leakage was less than that under hypoxia/reoxygenation, and such injury was inhibited by the green tea extract and the tannin mixture.