Assessing the relative contributions of phytochelatins and the cell wall to cadmium resistance in white lupin

Abstract

In response to Cd stress, higher plants utilise a number of defence systems, such as retention in cell walls, binding by organic molecules in the cytosol and sequestration in the vacuole. White lupin is a Cd‐resistant legume that is of interest for phytoremediation of acidified and Cd‐contaminated soils. The aim of this research was to evaluate the contributions of various mechanisms of Cd detoxification used by this species, focusing on cell‐wall retention and binding by thiol‐rich compounds. Retention of Cd by the cell wall of white lupin was well described by a Langmuir isotherm model. The percentage of total Cd adsorbed by the cell wall ranged from 29 to 47% in leaves, from 38 to 51% in stems and from 26 to 42% in roots depending on the Cd supply. Cadmium induced the synthesis of high levels of phytochelatins (PCs) in lupin plants, mainly in roots, with PC 3 being the major PC. The amount of Cd complexed by thiols accounted for approximately 20% of the total Cd in leaves, 40% in stems and 20% in roots. Therefore, cell‐wall retention could account for more than twice the amount of Cd complexed by PCs in leaves and roots. In stems, both mechanisms contributed equally to Cd detoxification. These studies indicate that white lupin plants use cell‐wall binding and, secondarily, the production of PCs, as effective mechanisms of Cd detoxification.