hGFAP‐cre transgenic mice for manipulation of glial and neuronal function in vivo

TLDR

The authors generated a transgenic mouse line expressing Cre recombinase under the human GFAP promoter to enable astrocyte‑specific gene modification. Cre activity was monitored by crossing the hGFAP‑Cre mice with lacZ reporter lines that express β‑galactosidase only after Cre‑mediated excision of a loxP‑flanked stop cassette. lacZ expression was largely restricted to the CNS, being widespread in neurons and ependymal cells but absent in cerebellar Purkinje neurons and choroid plexus epithelium, with Cre activation beginning in the forebrain by embryonic day 13.5, indicating hGFAP promoter activity in multipotent neural stem cells. © 2001 Wiley‑Liss, Inc.; genesis 31:85–94, 2001.

Abstract

Abstract With the goal of performing astrocyte‐specific modification of genes in the mouse, we have generated a transgenic line expressing Cre recombinase under the control of the human glial fibrillary acidic protein (hGFAP) promoter. Activity was monitored by crossing the hGFAP‐cre transgenics with either of two reporter lines carrying a lacZ gene whose expression requires excision of loxP‐flanked stop sequences. We found that lacZ expression was primarily limited to the central nervous system, but therein was widespread in neurons and ependyma. Cell types within the brain that notably failed to activate lacZ expression included Purkinje neurons of the cerebellum and choroid plexus epithelium. Onset of Cre expression began in the forebrain by e13.5, suggesting that the hGFAP promoter is active in a multi‐potential neural stem cell. genesis 31:85–94, 2001. © 2001 Wiley‐Liss, Inc.