Cytogenetics and Cell Genetics · 2008 · 41 citations · 0 references
Life SciencesGeneticsMolecular GeneticsCytogeneticsGenomicsMedical GeneticsGene MappingGenetic DisordersGenetic MappingIn Situ HybridizationMolecular CytogeneticsMolecular MarkersCdna CloningDna In Situ HybridizationFragile SitesHybridoma Cell LinesMolecular Hybridization
The human adenine phosphoribosyltransferase gene (APRT) was mapped with respect to the haptoglobin gene (HP) and the fragile site at 16q23.2 (FRA16D). A subclone of APRT and a cDNA clone of HP were used for molecular hybridization to DNA from mouse-human hybrid cell lines containing specific chromosome 16 translocations. The APRT subclone was used for in situ hybridization to chromosomes expressing FRA16D. APRT was found to be distal to HP and FRA16D and was localized at 16q24, making the gene order cen-FRA16B-HP-FRA16D-APRT-qter.